Technologie Hero Istock-695675608 Jaczhou

Methods

General Techniques


  • Derivation, expansion, manipulation and differentiation of stem cells
  • Genetic & Genomic Engineering
  • Analysis of immune cell activation
  • Animal model studies in cooperation with Charité, Universitätsmedizin Berlin/ Berlin-Brandenburg Centre for Regenerative Therapies (BCRT)/ German Heart Center Berlin (DHZB)

Methods


  • Cytotoxicity testing of polymer based biomaterials according to the DIN ISO 10993 (direct, indirect)
  • In-vitro-transcribed mRNA: synthesis and analysis
  • HPLC purification of nucleic acids
  • Gene delivery with Polymer-and Lipid-based carriers
  • Electroporation
  • Immunoblotting and Bioplex analysis
  • Reportergene assays
  • Cell engineering via retro-/lentiviruses (S2 facility)
  • Conditional gene expression technology
  • Flow cytomery (analytic and preparative)
  • Immune-assays
  • Transcriptome profiling
  • Hypoxia-cultivation of cells
  • Induced pluripotent stem cell culture
  • STED microscopy
  • Recombinant protein production